Depletion of epsilon-COP in the COPI Vesicular Coat Reduces Cleistothecium Production in Aspergillus nidulans
Mycobiology
;
: 31-36, 2015.
Article
Dans Anglais
| WPRIM
| ID: wpr-729750
ABSTRACT
We have previously isolated epsilon-COP, the alpha-COP interactor in COPI of Aspergillus nidulans, by yeast two-hybrid screening. To understand the function of epsilon-COP, the aneA+ gene for epsilon-COP/AneA was deleted by homologous recombination using a gene-specific disruption cassette. Deletion of the epsilon-COP gene showed no detectable changes in vegetative growth or asexual development, but resulted in decrease in the production of the fruiting body, cleistothecium, under conditions favorable for sexual development. Unlike in the budding yeast Saccharomyces cerevisiae, in A. nidulans, over-expression of epsilon-COP did not rescue the thermo-sensitive growth defect of the alpha-COP mutant at 42degrees C. Together, these data show that epsilon-COP is not essential for viability, but it plays a role in fruiting body formation in A. nidulans.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Aspergillus nidulans
/
Saccharomyces cerevisiae
/
Levures
/
Dépistage de masse
/
Protéine du coatomère
/
Développement sexuel
/
Saccharomycetales
/
Recombinaison homologue
/
Fruit
Type d'étude:
Étude de dépistage
langue:
Anglais
Texte intégral:
Mycobiology
Année:
2015
Type:
Article
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