Optimization of Protein Extraction for Lichen Thalli
Mycobiology
;
: 157-162, 2015.
Article
Dans Anglais
| WPRIM
| ID: wpr-729847
ABSTRACT
Lichen-forming fungal proteins have been seldom searched due to many difficulties in their extraction. Phenols, quinones, proteases, and other components released during cell disruption have been known to be the greatest challenges related to protein extraction from lichens. To overcome these problems and maintain good electrophoretic resolution and high protein concentration, an extraction buffer containing polyvinylpolypyrrolidone, ascorbic acid, Triton X-100, polyethylene glycol, proteinase, and oxidase inhibitors in sodium phosphate buffer was developed. This extraction buffer showed high efficiency for all lichen species tested in the study.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Oxidoreductases
/
Peptide hydrolases
/
Phénols
/
Polyéthylène glycols
/
Acide ascorbique
/
Quinones
/
Sodium
/
Protéines fongiques
/
Octoxinol
/
Phénol
langue:
Anglais
Texte intégral:
Mycobiology
Année:
2015
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS