Effects of knocking down Foxp3 on immunological functions of human neuroblastoma SK-N-BE2 cell / 中华实用儿科临床杂志

ABSTRACT

Objective To investigate whether knockingdown Foxp3 affects the immunological function of neuroblastoma cells.Methods Different siRNAs of Foxp3 were designed and the one that effectively inhibited Foxp3 gene expression was selected by real-time polymerase chain reaction and by fluorescence activated cell sorter analysis.Effects of Foxp3 knocking down on the expression of co-stimulatory molecule CD86 were also examined.Finally,neuroblastoma cells silenced of Foxp3 expression were co-cultured with human peripheral blood monouclear cells and the activities of T cells were examined.Results Expression of Foxp3 in SK-N-BE2 cells could be efficiently knocking down in both mRNA and protein levels.Knocking down Foxp3 increased the expression of CD86 and moderately increased IFN-γand IL-17 expressions,and significantly inhibited the expressions of TGF-β and IL-10.Conclusion Silencing Foxp3 expression in neuroblastoma SK-N-BE2 cells reverses its immune evasion mechanisms,which suggests that this is a new strategy for treating this kind of diseases.