Change of Toll-like receptors in children with acute Epstein-barr virus infection / 中华实用儿科临床杂志

ABSTRACT

Objective To investigate the possible role of Toll-like receptors (TLRs) in immunological pathogenesis of Epstein-barr virus(EBV) infection.Methods Fifteen children with acute EBV infection,18 children with infectious mononucleosis (IM),and 25 age-matched healthy children were enrolled in the study.Reverse-transcription polymerase chain reaction and real-time PCR were used to evaluate the levels of TLR2,TLR3,TLR7,TLR9,myeloid differentiation factor 88 (MyD88),tumor necrosis factor receptor-associated factor 6 (TRAF6),TGF-β activated kinase 1 (TAK1),TIR domain-containing adaptor protein inducing interferon β (TRIF),tumor necrosis factor receptor-associated factor 3 (TRAF3),TBK1,IL-1β,TNF-α,IFN-α and IFN-β mRNA expression in peripheral blood mononuclear cells(PBMC).The plasma concentrations of cytokines such as IL-12 and interferon gamma (IFN-γ) were determined by enzyme-linked immunosorbent assay.Results 1.Compared with healthy control group,the expression levels of TLR2,TLR3,TLR7,TLR9,MyD88,TRAF6,TAK1,TRIF,TRAF3 and TBK1 mRNA were up-regulated significantly from the children with acute EBV infection and IM (all P ＜ 0.05),there was no difference between the children with acute EBV infection and IM(all P ＞ 0.05).2.The levels of cytokines expression such as IL-1β,TNF-α,IFN-α and IFN-β in children with acute EBV infection were higher than those of the healthy control group (all P ＜0.05).3.The plasma concentrations of IL-12 and IFN-γwere up-regulated (all P ＜ 0.05) in children with acute EBV infection.Conclusion Imbalance of antiviral/inflammatory response resulting from the aberrant activation of TLRs may be one of the factors causing disturbed immunological function in vivo infected by EBV.