Role of Th17 cell and CD4+CD25+ regulatory T cells in inflammatory response in neonatal sepsis / 中华实用儿科临床杂志

ABSTRACT

Objective To investigate the roles of Thl7 cells and CD4 + CD25 + regulatory T cells in inflammatory response in neonatal sepsis.Methods Twenty children with neonatal sepsis (neonatal sepsis group) and 16 healthy neonates (healthy control group)were studied.Flow cytometric analysis (FCM) was performed to detect the percentage of CD4 + CD25 + Treg cells subpopulation.Real-time transcription-polymerase chain reaction (Real-time PCR) was used to analyze interleukin-17A (IL-17A),IL-17F,the transcription factor retinoid-related orphan nuclear receptor γt (ROR-γt),the forkhead/winged-helix protein 3 (Foxp3) and cytokines IL-6,transforming growth factor beta (TGF-β) expression in CD4 + T cell.Expressions of proinflammatory cytokines [IL-1β,IL-6,IL-10,tumor necrosis factor α (TNF-α)] were measured by using enzyme-linked immunosorbent serologic assay.Results Compared with healthy control group1.The proportions of CD4 + CD25 + Treg cells in neonatal sepsis group were significantly higher [(15.33 ± 2.68) ％ vs (2.96 ± 0.56) ％,P ＜ 0.01].The mRNA expression of transcription factor Foxp3 in neonatal sepsis group showed similar tendency[(42.76 ± 10.83) × 10-4 vs (22.34 ±4.17) × 10-4,P ＜0.01].2.Expression levels of IL-17A and IL-17F were significantly up-regulated in neonatal sepsis group[IL-17A(13.56 ±3.21) × 10-6 vs (4.76 ±1.39) ×10-6,P＜0.01 ;IL-17F(7.62 ±1.45) ×10-4 vs (1.89 ±0.48) ×10-4,P＜0.01] and the expression levels of the transcription factor ROR-γt in CD4 + T cells were significantly increased in neonatal sepsis group [(9.22 ± 1.79) × 10-5 vs (2.84 ±0.56) × 10-5,P ＜0.01].3.Expressions of proinflammatory cytokines (IL-1β,IL-6,IL-8,TNF-α) in neonatal sepsis group were higher than those in control group [IL-1β(2977.36 ± 653.97) pg/L vs (480.52 ± 120.36) pg/L,P ＜ 0.01 ; IL-6(3143.82 ± 775.08) pg/L vs (393.78 ± 96.55) pg/L,P ＜ 0.01) ; IL-10(3216.98 ± 678.43) pg/L vs (326.11 ± 62.45) pg/L,P ＜ 0.01 ; TNF-α(3582.24 ± 876.13) pg/L vs (1233.68 ± 289.39) pg/L,P ＜ 0.01].Conclusion Aberrant activation of Th17 cell and CD4 + CD25 + Treg cell might be involved in pathogenesis in neonatal sepsis.