Effect of bupivacaine on mEPSCs and mIPSCs of neurons in hippocampal CA1 region of rats / 中华麻醉学杂志

ABSTRACT

Objective To evaluate the effect of bupivacaine on miniature excitatory postsynaptic currents (mEPSCs) and miniature inhibitory postsynaptic currents (mIPSCs) of neurons in hippocampal CA1 region of rats. Methods Twenty SPF healthy male Sprague-Dawley rats, aged 4-6 weeks, weighing 300-350 g, were divided into control group ( C group, n=10) and bupivacaine model group ( BPV group, n=10) using a random number table method. The central nervous system toxicity model was established by infusing 0. 75％ bupivacaine at a rate of 1 mg·kg-1 ·min-1 via the tail vein in BPV group, while normal saline was infused for 7. 5 min at a rate of 1 mg·kg-1 ·min-1 via the tail vein in C group. Rats were sacri-ficed at 6 h after successful establishment of the model, the brains was removed and hippocampal slices were prepared. The whole-cell patch-clamp technique was used to record the frequency and amplitude of mEPSCs and mIPSCs. Results Compared with C group, the frequency of mIPSCs was significantly de-creased ( P<0. 01) , and no significant change was found in the amplitude of mEPSCs or frequency and am-plitude of mIPSCs in BPV group ( P>0. 05) . Conclusion The mechanism of bupivacaine-induced central nervous system toxicity is related to decreasing the frequency of mIPSCs in hippocampal neurons of rats.