Delineation of chromosome translocations by fluorescence in situ hybridization / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 853-856, 2000.
Article
Dans Chinois
| WPRIM
| ID: wpr-735319
ABSTRACT
Objective:
To delineate the G-banding-sug gested chromosome translocations by fluorescence in situ hybridization (FISH ) technique.Methods:
Locus-specific probes, generated by degen erate oligonucleotide-primed PCR (DOP-PCR) technique from yeast artificial chr omosomes (YACs) mapping the regions in question, were used for FISH tests.Results:
Among the 2 cases unresolved by G-banding, FISH confirm ed that one had a balanced translocation between chromosome 11 and chromosome 13 , the other had an unbalanced translocation between chromosome 6 and chromosome X.Conclusion:
Because of its high sensitivity and specificity, FISH technique is a powerful adjunct to chromosome banding techniques, particula rly for the delineation of subtle chromosome rearrangement(s) and the origin of segment(s).
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Academic Journal of Second Military Medical University
Année:
2000
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS