Delineation of chromosome translocations by fluorescence in situ hybridization / 第二军医大学学报
Academic Journal of Second Military Medical University
; (12): 853-856, 2000.
Article
de Zh
| WPRIM
| ID: wpr-736787
Bibliothèque responsable:
WPRO
ABSTRACT
Objective:To delineate the G-banding-sug gested chromosome translocations by fluorescence in situ hybridization (FISH ) technique. Methods: Locus-specific probes, generated by degen erate oligonucleotide-primed PCR (DOP-PCR) technique from yeast artificial chr omosomes (YACs) mapping the regions in question, were used for FISH tests. Results: Among the 2 cases unresolved by G-banding, FISH confirm ed that one had a balanced translocation between chromosome 11 and chromosome 13 , the other had an unbalanced translocation between chromosome 6 and chromosome X.Conclusion: Because of its high sensitivity and specificity, FISH technique is a powerful adjunct to chromosome banding techniques, particula rly for the delineation of subtle chromosome rearrangement(s) and the origin of segment(s).
Texte intégral:
1
Indice:
WPRIM
langue:
Zh
Texte intégral:
Academic Journal of Second Military Medical University
Année:
2000
Type:
Article