Performance of the cobas u 701 Analyzer in Urinary Tract Infection Screening
Annals of Laboratory Medicine
;
: 464-469, 2019.
Article
Dans Anglais
| WPRIM
| ID: wpr-739144
ABSTRACT
BACKGROUND:
Negative urine cultures to rule out urinary tract infections (UTI) generate a considerable laboratory workload; thus, a rapid screening test is desirable. We evaluated the performance of a new automated microscopy analyzer, cobas u 701 (Roche Diagnostics International, Rotkreuz, Switzerland) for the screening of UTI, and developed a rule-out strategy to reduce the number of samples requiring culture. We also assessed squamous epithelial cell (SEC) count as a predictor of culture contamination.METHODS:
In total, 1,604 urine samples from outpatients were analyzed with cobas u 701 and culture. Bacterial (BAC) and white blood cell (WBC) counts were used for sample interpretation. To determine a useful cut-off point to predict negative cultures, we selected the highest sensitivity and specificity values obtained from ROC curves. Diagnostic accuracy by age and gender was evaluated.RESULTS:
Urine culture showed growth of ≥104 colony forming units (CFU)/mL in 256 samples (16.0%). The highest sensitivity (91.8%) and specificity (68.4%) were obtained for cut-off points of 119 BAC/µL and 22 WBC/µL. The combination of BAC and WBC improved the performance of the rule-out strategy with a low rate of false-negative results (1.5%) and a high negative predictive value (NPV, 97.3%). Fifty-seven percent of the samples would not have required culture. SEC count was a poor predictor of culture contamination.CONCLUSIONS:
cobas u 701 can substantially reduce the number of urine samples requiring culture, with a low false-negative rate and a high NPV.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Patients en consultation externe
/
Cellules souches
/
Voies urinaires
/
Infections urinaires
/
Dépistage de masse
/
Courbe ROC
/
Sensibilité et spécificité
/
Cellules épithéliales
/
Leucocytes
/
Microscopie
Type d'étude:
Etude diagnostique
/
Étude pronostique
/
Étude de dépistage
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Annals of Laboratory Medicine
Année:
2019
Type:
Article
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