Decellularization of Trachea With Combined Techniques for Tissue-Engineered Trachea Transplantation
Clinical and Experimental Otorhinolaryngology
;
: 86-94, 2019.
Article
Dans Anglais
| WPRIM
| ID: wpr-739226
ABSTRACT
OBJECTIVES:
The purpose of this study is to shorten the decellularization time of trachea by using combination of physical, chemical, and enzymatic techniques.METHODS:
Approximately 3.5-cm-long tracheal segments from 42 New Zealand rabbits (3.5±0.5 kg) were separated into seven groups according to decellularization protocols. After decellularization, cellular regions, matrix and strength and endurance of the scaffold were followed up.RESULTS:
DNA content in all groups was measured under 50 ng/mg and there was no significant difference for the glycosaminoglycan content between group 3 (lyophilization+deoxycholic acid+de-oxyribonuclease method) and control group (P=0.46). None of the decellularized groups was different than the normal trachea in tensile stress values (P>0.05). Glucose consumption and lactic acid levels measured from supernatants of all decellularized groups were close to group with cells only (76 mg/dL and 53 mg/L).CONCLUSION:
Using combination methods may reduce exposure to chemicals, prevent the excessive influence of the matrix, and shorten the decellularization time.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Trachée
/
ADN
/
Acide lactique
/
Ingénierie tissulaire
/
Acide désoxycholique
/
Lyophilisation
/
Glucose
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Clinical and Experimental Otorhinolaryngology
Année:
2019
Type:
Article
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