Anti-Migration and Anti-Invasion Effects of Curcumin via Suppression of Fascin Expression in Glioblastoma Cells
Brain Tumor Research and Treatment
; : 16-24, 2019.
Article
Dans En
| WPRIM
| ID: wpr-739670
Responsable en Bibliothèque :
WPRO
ABSTRACT
BACKGROUND: The natural compound curcumin was known to inhibit migration and invasion of glioblastoma (GBM) cells. Fascin, a kind of actin-binding proteins, is correlated with migration and invasion of GBM cells. The purpose of this study was to investigate anti-migration and anti-invasion effects of curcumin via suppression of fascin expression in GBM cells. METHODS: U87 cell line was used as an experimental model of GBM. Fascin was quantified by Western blot analysis. And, the signal transducer and activator of transcription 3 (STAT3), known to play an important role in migration and invasion of tumor cells, were analyzed by sandwich-ELISA. Migration and invasion capacities were assessed by attachment, migration and invasion assays. Cellular morphology was demonstrated by immunofluorescence. RESULTS: At various concentrations of curcumin and exposure times, fascin expression decreased. After temporarily exposure to 10 µM/L curcumin during 6 hours as less invasive concentration and time, fascin expression temporarily decreased at 12 hours (18.4%, p=0.024), and since then recovered. And, the change of phosphrylated STAT3 level also reflected the temporarily decreased pattern of fascin expression at 12 hours (19.7%, p=0.010). Attachment, migration, and invasion capacities consistently decreased at 6, 12, and 24 hours. And, immunofluorescence showed the change of shape and the reduction of filopodia formation in cells. CONCLUSION: Curcumin is likely to suppress the fascin expression in GBM cells, and this might be a possible mechanism for anti-migration and anti-invasion effects of Curcumin via inhibition of STAT3 phosphorylation.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phosphorylation
/
Pseudopodes
/
Lignée cellulaire
/
Technique de Western
/
Technique d'immunofluorescence
/
Glioblastome
/
Curcumine
/
Émigration et immigration
/
Facteur de transcription STAT-3
/
Protéines des microfilaments
Type d'étude:
Prognostic_studies
langue:
En
Texte intégral:
Brain Tumor Research and Treatment
Année:
2019
Type:
Article