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Expression and Localization of Keap1 During Amelogenesis in the Developing Molar Germ of Rats
International Journal of Oral Biology ; : 177-183, 2018.
Article Dans Anglais | WPRIM | ID: wpr-740086
ABSTRACT
The objective of this study was to examine the expression pattern of Kelch-like ECH-associated protein 1 (Keap1) in the maxillary 2(nd) molar germs of rats. We used the maxillary 2(nd) molar germs in rats' pup at postnatal day 3 (bell stage), 6 (crown formation stage) and 9 (root formation stage). The investigation on mRNA and protein levels were done using reverse transcription - polymerase chain reaction and western blot. Localization of Keap 1 in the maxillary 2(nd) molar germs were revealed through immunofluorescence staining. Keap1 from the maxillary 2nd molar germs were mostly manifested on postnatal day 3 and dramatically decreased on postnatal day 6 and 9 at mRNA and protein levels, while amelogenin and ameloblastin increased during the development of maxillary 2nd molar germs. During immunofluorescence analysis, the strong immunoreactivity against Keap1 was detected in the apical side of ameloblasts at the presecretory and secretory stages. However, Keap1 expression was hardly observed in the ameloblasts at the maturation stage. These results shows that Keap1 is strongly expressed in the presecretory and secretory ameloblasts of amelogenesis, and suggest that Keap1 may be a crucial molecule for the regulatory mechanisms tasked with the formation of enamel layer.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: ARN messager / Technique de Western / Réaction de polymérisation en chaîne / Technique d'immunofluorescence / Émail dentaire / Transcription inverse / Amélogénine / Améloblastes / Amélogenèse / Molaire Limites du sujet: Animaux langue: Anglais Texte intégral: International Journal of Oral Biology Année: 2018 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: ARN messager / Technique de Western / Réaction de polymérisation en chaîne / Technique d'immunofluorescence / Émail dentaire / Transcription inverse / Amélogénine / Améloblastes / Amélogenèse / Molaire Limites du sujet: Animaux langue: Anglais Texte intégral: International Journal of Oral Biology Année: 2018 Type: Article