Identification and HPLC Quantification of a Phytoecdysone and Three Phenolic Glycosides in Lamium takesimense Nakai
Natural Product Sciences
;
: 241-246, 2018.
Article
Dans Anglais
| WPRIM
| ID: wpr-741635
ABSTRACT
The herbs of Lamium takesimense Nakai (Lamiaceae) is used to treat spasmodic and inflammatory disease. The four polar compounds, ecdysterone, isoacteoside, rutin and lamiuside C, were isolated and identified from the BuOH fraction of the L. takesimense MeOH extract. HPLC quantification was performed on a Capcell Pak C18 column (5 µm, 4.6 mm × 250 mm) with a gradient elution of H₂O and 0.05% acetic acid in MeOH. The HPLC method was validated in terms of linearity, sensitivity, stability, precision, and accuracy. The quantitative level in plant material was determined as the following order lamiuside C (4, 3.75 mg/g dry weight) > ecdysterone (1, 1.93 mg/g) > isoacteoside (2, 1.32 mg/g) > rutin (3, 0.97 mg/g).
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Plantes
/
Rutoside
/
Chromatographie en phase liquide à haute performance
/
Acide acétique
/
Lamiaceae
/
Phénol
/
Ecdysone
/
Ecdystérone
/
Hétérosides
/
Méthodes
Type d'étude:
Etude diagnostique
langue:
Anglais
Texte intégral:
Natural Product Sciences
Année:
2018
Type:
Article
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