Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep
The Korean Journal of Parasitology
;
: 61-67, 2019.
Article
Dans Anglais
| WPRIM
| ID: wpr-742303
ABSTRACT
Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Maladies parasitaires
/
Test ELISA
/
Tests sérologiques
/
Ovis
/
Sensibilité et spécificité
/
Déterminants antigéniques des lymphocytes B
/
Echinococcus granulosus
/
Échinococcose
/
Animaux domestiques
/
Méthodes
Type d'étude:
Etude diagnostique
langue:
Anglais
Texte intégral:
The Korean Journal of Parasitology
Année:
2019
Type:
Article
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