Effect of taurine on the antioxidant capacity and ATP activity of skeletal muscle in rats with exhaustive exercise / 中国基层医药

ABSTRACT

Objective To explore the effect of taurine on the activity of SOD,MDA,Na +-K +-ATP enzyme and Bax/Bcl-2 in skeletal muscle of rats after exhaustive exercise.Methods Thirty male SD rats (6 months old) were randomly divided into normal group,control group and taurine group,with 10 rats in each group.The normal group was given routine feeding for 1 week,adaptive training for 3 days,without applying other measures.The control group was given routine feeding for 1 week,after adaptive training for 3 days underwent exhaustive exercise for 2 days.The taurine group was given 200mg/kg taurine gavage daily on the basis of the control group.The activity of SOD,MDA,Na+-K+-ATP and Bax/Bcl-2 were measured in the skeletal muscle after 2 days exhaustive exercise.Results After 2 days exhaustive exercise,the SOD concentration in skeletal muscle of the control group was (146.58 ± 13.42) U/mg prot,which was lower than that in the taurine group [(143.81 ± 15.93) U/mg prot] (t =2.519,P ＜0.05).The MDA concentration in the skeletal muscle of the control group was (1.97 ± 0.20) nmol/mg prot,which was higher than that in the taurine group [(1.22 ± 0.19) nmol/mg prot] (t =2.356,P ＜ 0.05).The ratio of SOD/MDA in the control group was (60.86 ±20.38),which was lower than that in the taurine group [(120.87 ±23.51)] (t =4.071,P ＜ 0.05).The activity of Na +-K +-ATP in the control group was (2.42 ± 0.67) U/mg prot,which was lower than that in the taurine group [(5.74 ± 1.15) U/mg prot] (t =3.905,P ＜ 0.05).The ratio of Bax/Bcl-2 in the control group was (1.62 ± 0.17),which was higher than that in the taurine group [(0.96 ±0.14)] (t=3.419,P ＜ 0.05).Conclusion Taking taurine before exhaustive exercise can increase the ability of scavenging free radicals,reduce the production of oxidative stress products and protect the activity of Na +-K +-ATP enzyme.It is benefit for maintain the stability of cell environment and prevent skeletal muscle injury.