Effects of ultrashortwave irradiation on the adhesion, proliferation and osteogenic differentiation of mesenchymal stem cells / 中华物理医学与康复杂志

ABSTRACT

Objective To observe the effect of ultrashortwave irradiation on the adhesion,proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (rMSCs) in rats.Methods The rMSCs were put under ultrashortwave radiation for 15 minutes every day.Early cell adhesion was detected after 1 day of the treatment.The cell morphology was observed under an inverted fluorescence microscope on the 3rd day,the cell proliferation was measured on the 1st,3rd and 7th day,while the expression of the osteogenic gene osteopontin was detected on the 8th day.Results Early cell adhesion increased 80％ (a significant increase) and more pseudo-feet with a larger range of spread were observed.After 7 days of irradiation the proliferation and the expression of osteogenic gene osteopontin had both increased significantly.Conclusion Ultrashortwave irradiation can affect the early adhesion and morphology of rMSCs and promote their proliferation and osteogenic differentiation.