The effect of short hairpin RNA and DNAzyme gene therapy on latent membrane protein-1 expression in nasopharyngeal carcinoma / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 270-272, 2010.
Article
de Zh
| WPRIM
| ID: wpr-746616
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE@#To study the inhibitory effects of short hairpin RNA (pshLMP1) combined with DNAzyme or alone on latent membrane protein-1 (LMP1)expression in HNE1 cell lines.@*METHOD@#The pshLMP1 and DNAzymes were co-transfected with pEGFP-N1-1158 (a expression vector encoding LMP1 and EGFP fusion protein) into HNE1 cells, divided into 4 groups as positive control, RNA interference, combining and DNAzyme groups. The green fluorescence were analyzed and then the mRNA and protein of LMP1 gene were detected.@*RESULT@#The HNE1 cells expressing green fluorescence in combining group were significantly less than that in RNA interference group (P < 0.01); The inhibition efficiency of green fluorescence in combining group were 86. 31% Wp 88.88% ,which were higher than that in RNA interference group with 75.15%. The detection of LMP1 mRNA and proteins showed that combining group had a higher inhibition ability.@*CONCLUSION@#Combining gene therapy with pshLMP1 and DNAzyme is superior to pshLMP1 alone to inhibit LMP1 gene expression.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
/
Transfection
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Thérapie génétique
/
Régulation de l'expression des gènes viraux
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Protéines de la matrice virale
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Tumeurs du rhinopharynx
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Exons
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ADN catalytique
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Petit ARN interférent
/
Lignée cellulaire tumorale
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Année:
2010
Type:
Article