Effect of tanshinone II A to B7H3 expression in human nasopharyngeal carcinoma CNE1 cell lines / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#To explore the proliferation of human nasopharyngeal carcinoma CNE1 cell and B7H3 expiration after treating by Tanshinone II A (Tan II A).@*METHOD@#The CNE1 cell was cultured in vitro and treated by Tan II A with different concentrations; Cell proliferation was valuated by MTT; Apoptotic rate and cell cycle were investigated by flow cytometry; Bcl2 and B7H3 expiration were detected by direct immunofluorescence assay.@*RESULT@#The CNE1 cell's proliferation has been significantly inhibited by Tan II A, the inhibition was in dose and time effect with Tan II A, with significant difference (P<0.01); IC50 value in 24, 48 and 72 hours was respective 12.5 micromol/L, 4.8 micromol/L and 3.0 micromol/L; Comparing with the control group, Tan II A group increased apoptosis rate (P<0.01); Cell cycle was blocked in G2/M phase; Bcl2 and B7H3 expression were more decreased in Tan II A group than in control group with significant difference (P<0.05);@*CONCLUSION@#Tan II A caused obviously growth inhibitory effect and induced apoptosis in CNE1 cells and the mechanisms might be related to down regulation of Bcl2 and B7H3.