Detection and correlation of lipopolysaccharide, vitamin D receptor and matrix metalloproteinase-9 in the middle ear cholesteatoma / 临床耳鼻咽喉头颈外科杂志

ABSTRACT

OBJECTIVE@#To investigate the expression and concentration of lipopolysaccharide (LPS) and matrix metalloproteinase-9 (MMP-9) in middle ear cholesteatoma and discuss their relations.@*METHOD@#Twenty-nine cases of middle ear cholesteatoma tissue, 18 cases of external auditory canal tissue were detected by limulus amebocyte lysate assay (LAL-assay), and expression of MMP-9 protein in formalin-fixed, paraffin-embedded tissues was detected by immunohistochemical method.@*RESULT@#The concentrations of LPS in cholesteatoma were higher than that in external auditory canal tissues. In group of cholesteatoma M = 0.739 0, IQR = 0.6203, and in group of external auditory canal tissues M = -0.2538, IQR = 1.1692 (P 0.05). LPS, MMP-9 weren't significantly correlated by Spearman test.@*CONCLUSION@#LPS was responsible for middle ear cholesteatoma and its related bone erosion. MMP-9 was related to the development of middle ear cholesteatoma. There's no correlation between LPS and MMP-9.