Investigation of self-renewal mechanism about CD133+ cancer stem cells in human laryngeal carcinoma Hep-2 cell line / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
; (24): 1636-1641, 2014.
Article
de Zh
| WPRIM
| ID: wpr-747676
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the self-renewal mechanism of CD133+ cancer stem cells from Hep-2 cell line.@*METHOD@#The CD133+ cells were sorted by flow cytometry from Hep-2 cell line. Then the sorted CD133+ cells were cultured in RPMI1640. The ability of self-renewal of CD133+ cells were tested by MTT assay. mRNA and protein expression of self-renewal related genes were detected by western blot and RT- PCR.@*RESULT@#(3.10 ± 0.21)% of Hep-2 cells expressed the membrane antigen CD133. CD133+ fraction was raised to (90.20 ± 5.51)% by flow cytometry. In vitro culture and growth curve showed CD133+ cells had more active proliferation ability than CD133- cells, which showed statistically significant difference between these two group (P < 0.01). RT- PCR and western blot results showed upregulated mRNA and protein expression of Fas, c-myc, survivin in CD133+ group (P < 0.01). In the same time, the ratio of Bcl-2/Bax gene expression was obviously increased in CD133+ group. Self-renewal related gene such as β-catenin, SHH, SMOH and Bmi-1,Gli-1 were all up-regulated in CD133+ group both in mRNA and protein. On the contrary, PTCH gene was down-regulated.@*CONCLUSION@#CD133 positive cells are a small proportion of a Hep-2 cell line. The results of this experiment verified that CD133 positive cells owned the properties of cancer stem cells. Upregulated anti-apoptotic gene is the foundatiom of self-renewal mechanism of CD133+ cells. Cancer stem cells related signal pathways such as Hedgehog, Wnt and Bmi-1 pathway are in state of activation. The identification of self-renewal mechanism about cancer stem cell provides a powerful tool to investigate the tumorigenic process in the larynx and to develop therapies targeting to these signal pathways.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Peptides
/
Physiologie
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Cellules souches tumorales
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Glycoprotéines
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Transduction du signal
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Antigènes CD
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Régulation négative
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Phénomènes physiologiques cellulaires
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Tumeurs du larynx
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Apoptose
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Clinical Otorhinolaryngology Head and Neck Surgery
Année:
2014
Type:
Article