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Effect of the combination of hyperbaric oxygen and 5-fluorouracil on the proliferation and metastasis of nasopharyngeal carcinoma CNE-2Z cell line / 临床耳鼻咽喉头颈外科杂志
Journal of Clinical Otorhinolaryngology Head and Neck Surgery ; (24): 386-391, 2010.
Article Dans Chinois | WPRIM | ID: wpr-747994
ABSTRACT
OBJECTIVE@#To investigate the effect and the possible influential mechanisms of hyperbaric oxygen (HBO) and 5-fluorouracil (5-FU) on the proliferation and metastasis in human nasopharyngeal carcinoma (NPC) CNE2Z cell line.@*METHOD@#NPC CNE2Z cell line were divided into 4 groups randomly group A control group; group B 5-FU group; group C HBO group; group D 5-FU and HBO group. The inhibition effect of proliferation in CNE2Z cells of all groups through 24 h, 48 h and 72 h disposal was detected by MTT reduction assay; Transwell chamber assay was performed to determine the effect of HBO and (or) 5-FU on the metastasis of the CNE2Z cells; The MMP-9, VEGF expression in CNE2Z cells of all groups were detected by SP immunocytochemical stain and observe the expressed image by micro.@*RESULT@#There were statistical difference on the inhibition effect of proliferation in C group and A group after 48 h and 72 h disposal (P0.05) and between B, C group and D group (P>0.05); Average optical of the MMP-9, VEGF expression were not statistically significant difference in C group and A group (P>0.05) and between C group and D group (P>0.05).@*CONCLUSION@#Simple HBO disposal after 48 h and 72 h could inhibit the proliferation potential of NPC CNE2Z cell line, but the combination of HBO and 5-FU only after 48 h disposal could all the more inhibit the proliferation potential. Simple HBO disposal couldn't decrease the MMP-9 and VEGF high-expression and inhibit the metastasis potential in human NPC CNE2Z cell line, the combination of HBO and 5-FU disposal also couldn't further decreased the MMP-9 and VEGF high-expression and inhibited the metastasis potential.
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Thérapeutique / Tumeurs du rhinopharynx / Apoptose / Matrix metalloproteinase 9 / Lignée cellulaire tumorale / Facteur de croissance endothéliale vasculaire de type A / Utilisations thérapeutiques / Prolifération cellulaire / Fluorouracil Limites du sujet: Humains langue: Chinois Texte intégral: Journal of Clinical Otorhinolaryngology Head and Neck Surgery Année: 2010 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Thérapeutique / Tumeurs du rhinopharynx / Apoptose / Matrix metalloproteinase 9 / Lignée cellulaire tumorale / Facteur de croissance endothéliale vasculaire de type A / Utilisations thérapeutiques / Prolifération cellulaire / Fluorouracil Limites du sujet: Humains langue: Chinois Texte intégral: Journal of Clinical Otorhinolaryngology Head and Neck Surgery Année: 2010 Type: Article