Sampling and storage methods of fecal samples in human intestinal microbiome study / 国际儿科学杂志

ABSTRACT

With the rapid development of high-throughput sequencing ( NGS) technique, intestinal microbiome could be studied more deeply. Intestinal contents and fecal samples have the characteristics of con-venient sampling and strong representation, so they are often used as the main research objects in the study of intestinal microbiota. The method of collecting and storage of samples are very important to affect the internal flora structure and diversity, which determines the accuracy of subsequent sequencing analysis. This review summarizes the sampling and storage methods of fecal samples in the study of intestinal microbiome.