Differentiation types of microglia induced by macrophage colony-stimulating factor: an in vitro cell experiment / 中华麻醉学杂志
Chinese Journal of Anesthesiology
;
(12): 206-208, 2019.
Article
Dans Chinois
| WPRIM
| ID: wpr-755521
ABSTRACT
Objective To study the differentiation types of microglia induced by macrophage colony-stimulating factor (M-CSF).Methods Rat microglia cultured in vitro were inoculated on 6-well plates and divided into 3 groups (n=4 each) using a random number table method when cell confluence reached 70%blank control group (C group),vehicle control group (P group) and M-CSF group.Group P was incubated with phosphate buffer solution for 7 days and group M-CSF with 20 ng/ml M-CSF for 7 days.The expression of a specific M1 phenotype marker tumor necrosis factor-alpha (TNF-α) and specific M2 phenotype markers interleukin-10 (IL-10) and brain-derived neurotrophic factor (BDNF) was determined by Western blot.Results Compared with C group,the expression of IL-10 and BDNF was significantly upregulated (P<0.05),and no significant change was found in TNF-α expression in M group (P>0.05),and no significant change was found in the expression of TNF-α,IL-10 or BDNF in P group (P>0.05).Conclusion M-CSF can induce microglia to differentiate into a M2 phenotype.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Anesthesiology
Année:
2019
Type:
Article
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