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A new method for quantifying mitochondrial axonal transport
Protein & Cell ; (12): 804-819, 2016.
Article Dans Anglais | WPRIM | ID: wpr-757370
ABSTRACT
Axonal transport of mitochondria is critical for neuronal survival and function. Automatically quantifying and analyzing mitochondrial movement in a large quantity remain challenging. Here, we report an efficient method for imaging and quantifying axonal mitochondrial transport using microfluidic-chamber-cultured neurons together with a newly developed analysis package named "MitoQuant". This tool-kit consists of an automated program for tracking mitochondrial movement inside live neuronal axons and a transient-velocity analysis program for analyzing dynamic movement patterns of mitochondria. Using this method, we examined axonal mitochondrial movement both in cultured mammalian neurons and in motor neuron axons of Drosophila in vivo. In 3 different paradigms (temperature changes, drug treatment and genetic manipulation) that affect mitochondria, we have shown that this new method is highly efficient and sensitive for detecting changes in mitochondrial movement. The method significantly enhanced our ability to quantitatively analyze axonal mitochondrial movement and allowed us to detect dynamic changes in axonal mitochondrial transport that were not detected by traditional kymographic analyses.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Physiologie / Transport axonal / Logiciel / Expression des gènes / Cortex cérébral / Rat Sprague-Dawley / Microscopie confocale / Biologie cellulaire / Protéine FUS de liaison à l'ARN / Drosophila melanogaster Limites du sujet: Animaux langue: Anglais Texte intégral: Protein & Cell Année: 2016 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Physiologie / Transport axonal / Logiciel / Expression des gènes / Cortex cérébral / Rat Sprague-Dawley / Microscopie confocale / Biologie cellulaire / Protéine FUS de liaison à l'ARN / Drosophila melanogaster Limites du sujet: Animaux langue: Anglais Texte intégral: Protein & Cell Année: 2016 Type: Article