A new unconventional HLA-A2-restricted epitope from HBV core protein elicits antiviral cytotoxic T lymphocytes
Protein & Cell
;
(12): 317-327, 2014.
Article
Dans Anglais
| WPRIM
| ID: wpr-757497
ABSTRACT
Cytotoxic T cells (CTLs) play a key role in the control of Hepatitis B virus (HBV) infection and viral clearance. However, most of identified CTL epitopes are derived from HBV of genotypes A and D, and few have been defined in virus of genotypes B and C which are more prevalent in Asia. As HBV core protein (HBc) is the most conservative and immunogenic component, in this study we used an overlapping 9-mer peptide pool covering HBc to screen and identify specific CTL epitopes. An unconventional HLA-A2-restricted epitope HBc141-149 was discovered and structurally characterized by crystallization analysis. The immunogenicity and anti-HBV activity were further determined in HBV and HLA-A2 transgenic mice. Finally, we show that mutations in HBc141-149 epitope are associated with viral parameters and disease progression in HBV infected patients. Our data therefore provide insights into the structure characteristics of this unconventional epitope binding to MHC-I molecules, as well as epitope specific CTL activity that orchestrate T cell response and immune evasion in HBV infected patients.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Liaison aux protéines
/
Sites de fixation
/
Souris transgéniques
/
Lymphocytes T cytotoxiques
/
Antigène HLA-A2
/
Chimie
/
Virus de l'hépatite B
/
Séquence d'acides aminés
/
Structure tertiaire des protéines
/
Allergie et immunologie
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Protein & Cell
Année:
2014
Type:
Article
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