MicroRNA-495 induces breast cancer cell migration by targeting JAM-A
Protein & Cell
;
(12): 862-872, 2014.
Article
Dans Anglais
| WPRIM
| ID: wpr-757649
ABSTRACT
MicroRNAs (miRNAs) are small, non-coding RNAs that function as post-transcriptional regulators of gene expression. The deregulated expression of miRNAs is associated with a variety of diseases, including breast cancer. In the present study, we found that miR-495 was markedly up-regulated in clinical breast cancer samples by quantitative real time-PCR (qRT-PCR). Junctional adhesion molecule A (JAM-A) was predicted to be a potential target of miR-495 by bioinformatics analysis and was subsequently verified by luciferase assay and Western blotting. JAM-A was found to be negatively correlated with the migration of breast cancer cells through loss-of-function and gain-of-function assays, and the inhibition of JAM-A by miR-495 promoted the migration of MCF-7 and MDA-MB-231 cells. Furthermore, overexpression of JAM-A could restore miR-495-induced breast cancer cell migration. Taken together, our findings suggest that miR-495 could facilitate breast cancer progression through the repression of JAM-A, making this miRNA a potential therapeutic target.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Tumeurs du sein
/
Molécules d'adhérence cellulaire
/
Régulation de l'expression des gènes tumoraux
/
Mouvement cellulaire
/
Technique de Western
/
Récepteurs de surface cellulaire
/
Régions 3' non traduites
/
RT-PCR
/
MicroARN
Type d'étude:
Étude pronostique
Limites du sujet:
Adulte
/
Adulte très âgé
/
Femelle
/
Humains
langue:
Anglais
Texte intégral:
Protein & Cell
Année:
2014
Type:
Article
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