Expression of YB-1 enhances production of murine leukemia virus vectors by stabilizing genomic viral RNA
Protein & Cell
;
(12): 943-949, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-757836
ABSTRACT
Murine leukemia virus (MLV)-based retroviral vectors is widely used for gene transfer and basic research, and production of high-titer retroviral vectors is very important. Here we report that expression of the Y-box binding protein 1 (YB-1) enhanced the production of infectious MLV vectors. YB-1 specifically increased the stability of viral genomic RNA in virus-producing cells, and thus increasing viral RNA levels in both producer cells and virion particles. The viral element responsive to YB-1 was mapped to the repeat sequence (R region) in MLV genomic RNA. These results identified YB-1 as a MLV mRNA stabilizer, which can be used for improving production of MLV vectors.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Virion
/
ARN viral
/
Séquence nucléotidique
/
Séquences répétées d'acides nucléiques
/
Génie génétique
/
Expression des gènes
/
Chimie
/
Génome viral
/
Virus de la leucémie murine
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Protein & Cell
Année:
2012
Type:
Article
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