Development and Clinical Evaluation of a Rapid Diagnostic Test for Yellow Fever Non-Structural Protein 1
The Korean Journal of Parasitology
;
: 283-290, 2019.
Article
Dans Anglais
| WPRIM
| ID: wpr-761741
ABSTRACT
A rapid diagnostic test (RDT) kit was developed to detect non-structural protein 1 (NS1) of yellow fever virus (YFV) using monoclonal antibody. NS1 protein was purified from the cultured YFV and used to immunize mice. Monoclonal antibody to NS1 was selected and conjugated with colloidal gold to produce the YFV NS1 RDT kit. The YFV RDTs were evaluated for sensitivity and specificity using positive and negative samples of monkeys from Brazil and negative human blood samples from Korea. Among monoclonal antibodies, clones 3A11 and 3B7 proved most sensitive, and used for YFV RDT kit. Diagnostic accuracy of YFV RDT was fairly high; Sensitivity was 0.0% and specificity was 100% against Dengue viruses type 2 and 3, Zika, Chikungunya and Mayaro viruses. This YFV RDT kit could be employed as a test of choice for point-of-care diagnosis and large scale surveys of YFV infection under clinical or field conditions in endemic areas and on the globe.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Fièvre jaune
/
Virus de la fièvre jaune
/
Brésil
/
Sensibilité et spécificité
/
Clones cellulaires
/
Haplorhini
/
Or colloïdal
/
Systèmes automatisés lit malade
/
Virus de la dengue
/
Diagnostic
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
/
Humains
Pays comme sujet:
Amérique du Sud
/
Asie
/
Brésil
langue:
Anglais
Texte intégral:
The Korean Journal of Parasitology
Année:
2019
Type:
Article
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