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The novel latex agglutination turbidimetric immunoassay system for simultaneous measurements of calprotectin and hemoglobin in feces
Intestinal Research ; : 202-209, 2019.
Article Dans Anglais | WPRIM | ID: wpr-764139
ABSTRACT
BACKGROUND/

AIMS:

Fecal calprotectin (Fcal) as well as the fecal immunochemical test (FIT) are useful biomarkers for detecting activity and mucosal healing in inflammatory bowel diseases. Here, we report the performance of simultaneous measurements of Fcal and FIT for ulcerative colitis (UC) patients using the newly-developed latex agglutination turbidimetric immunoassay (LATIA) system.

METHODS:

Fcal and hemoglobin were measured by the LATIA system in 152 UC patients who underwent colonoscopy. Fcal was also quantified with a conventional enzyme-linked immunosorbent assay (ELISA). Fecal markers were evaluated in conjunction with the mucosal status of UC, which was assessed via the Mayo endoscopic subscore (MES) classification.

RESULTS:

The LATIA system could quantify calprotectin and hemoglobin simultaneously with the same fecal samples within 10 minutes. The values of the Fcal-LATIA closely correlated with those of the Fcal-ELISA (Spearman rank correlation coefficient, r=0.84; P<0.0001). The values of Fcal for each assay and the FIT all significantly correlated with the MESs (Spearman rank correlation coefficient, Fcal-LATIA r=0.58, Fcal-ELISA r=0.55, and FIT r=0.72). The mucosal healing predictability (determined by an MES of 0 alone) of the Fcal-LATIA, Fcal-ELISA, and FIT-LATIA with the cutoffs determined by receiver operating characteristic curve analysis was 0.79, 0.78, and 0.92 for sensitivity, respectively, and 0.78, 0.69, and 0.73 for specificity, respectively.

CONCLUSIONS:

The performance of the novel Fcal-LATIA was equivalent to that of the conventional Fcal assay. Simultaneous measurements with FITs would promote the clinical relevance of fecal biomarkers in UC.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Test ELISA / Dosage immunologique / Marqueurs biologiques / Maladies inflammatoires intestinales / Rectocolite hémorragique / Courbe ROC / Sensibilité et spécificité / Coloscopie / Classification / Complexe antigénique L1 leucocytaire Type d'étude: Etude diagnostique / Étude pronostique Limites du sujet: Humains langue: Anglais Texte intégral: Intestinal Research Année: 2019 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Test ELISA / Dosage immunologique / Marqueurs biologiques / Maladies inflammatoires intestinales / Rectocolite hémorragique / Courbe ROC / Sensibilité et spécificité / Coloscopie / Classification / Complexe antigénique L1 leucocytaire Type d'étude: Etude diagnostique / Étude pronostique Limites du sujet: Humains langue: Anglais Texte intégral: Intestinal Research Année: 2019 Type: Article