Minimal Requirement of Limbal Epithelium for Successful Limbal Cell Transplantation in Rabbit Corneas
Journal of the Korean Ophthalmological Society
;
: 613-620, 2006.
Article
Dans Coréen
| WPRIM
| ID: wpr-76580
ABSTRACT
PURPOSE:
To investigate the minimal requirements of the limbal epithelium for successful limbal stem cell transplantation and the healing process.METHODS:
Nine rabbits were divided into 4, 6, and 8 clock-hour transplantation groups. Limbal autografts from the healthy fellow eye were transplanted to the iatrogenic damaged eye. The amniotic membrane served as a stem cell niche. Experimental corneas were evaluated by slit lamp examination and immunohistochemistry.RESULTS:
In the over 9 hours transplantation group, the healing process of the epithelium from the limbal stem cell was revealed and cornea-specific keratin k3, transcription factor p63, and connexin 43 were detected by immunohistochemistry. The normal corneal epithelium was regenerated after 60 days postoperatively in the fellow donor eye.CONCLUSIONS:
Limbal cell transplantation of over 9 hours seems to be a safe and effective method in the treatment of severe ocular surface disorders. In addition, the donation of limbal epithelium for up to 8 hours did not affect the normal corneal regenerating capability.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Cellules souches
/
Donneurs de tissus
/
Facteurs de transcription
/
Immunohistochimie
/
Connexine 43
/
Transplantation cellulaire
/
Épithélium antérieur de la cornée
/
Cornée
/
Transplants
/
Transplantation de cellules souches
Limites du sujet:
Animaux
/
Humains
langue:
Coréen
Texte intégral:
Journal of the Korean Ophthalmological Society
Année:
2006
Type:
Article
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