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Minimal Requirement of Limbal Epithelium for Successful Limbal Cell Transplantation in Rabbit Corneas
Journal of the Korean Ophthalmological Society ; : 613-620, 2006.
Article Dans Coréen | WPRIM | ID: wpr-76580
ABSTRACT

PURPOSE:

To investigate the minimal requirements of the limbal epithelium for successful limbal stem cell transplantation and the healing process.

METHODS:

Nine rabbits were divided into 4, 6, and 8 clock-hour transplantation groups. Limbal autografts from the healthy fellow eye were transplanted to the iatrogenic damaged eye. The amniotic membrane served as a stem cell niche. Experimental corneas were evaluated by slit lamp examination and immunohistochemistry.

RESULTS:

In the over 9 hours transplantation group, the healing process of the epithelium from the limbal stem cell was revealed and cornea-specific keratin k3, transcription factor p63, and connexin 43 were detected by immunohistochemistry. The normal corneal epithelium was regenerated after 60 days postoperatively in the fellow donor eye.

CONCLUSIONS:

Limbal cell transplantation of over 9 hours seems to be a safe and effective method in the treatment of severe ocular surface disorders. In addition, the donation of limbal epithelium for up to 8 hours did not affect the normal corneal regenerating capability.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Cellules souches / Donneurs de tissus / Facteurs de transcription / Immunohistochimie / Connexine 43 / Transplantation cellulaire / Épithélium antérieur de la cornée / Cornée / Transplants / Transplantation de cellules souches Limites du sujet: Animaux / Humains langue: Coréen Texte intégral: Journal of the Korean Ophthalmological Society Année: 2006 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Cellules souches / Donneurs de tissus / Facteurs de transcription / Immunohistochimie / Connexine 43 / Transplantation cellulaire / Épithélium antérieur de la cornée / Cornée / Transplants / Transplantation de cellules souches Limites du sujet: Animaux / Humains langue: Coréen Texte intégral: Journal of the Korean Ophthalmological Society Année: 2006 Type: Article