Ex vivo Expansion of Human Natural Killer Cells from Blood Retained in a Disposable Platelet Apheresis Set / 대한수혈학회지
Korean Journal of Blood Transfusion
; : 28-37, 2012.
Article
de Ko
| WPRIM
| ID: wpr-76696
Bibliothèque responsable:
WPRO
ABSTRACT
BACKGROUND: Natural killer cells expanded from human peripheral blood (PB) have been used in cancer immunotherapy research. Although most research teams have access to human PB, it is necessary to find a source of blood that can be easily obtained. We have tested the possibility of using blood retained in a disposable platelet apheresis set as an alternative source, with special interest in expansion of NK cells for use in cancer immunotherapy research. METHODS: For expansion of NK cells, peripheral blood mononuclear cells (PBMCs) were isolated from an MCS+ platelet apheresis kit (Haemonetics, Braintree, USA) and PB from the same donor (n=7) and co-cultured with 100-Gy gamma ray-irradiated K562 cells expressing the 4-1BB ligand and membrane-bound IL-15 for three weeks in RPMI1640 medium in the presence of IL-2 and IL-15. Cytotoxicity was measured using WST-1 at 1:1, 2:1, and 4:1 effector-to-target (E:T) ratios for a period of four hours. RESULTS: Mean rate of expansion of NK cells was 1,097-fold and their purity was 94.4% from blood retained in a disposable platelet apheresis set; mean rate of expansion of NK cells was 953-fold and their purity was 92.0% from PB after a period of three weeks. No differences in cytotoxicity against K562, 697, Raji, and RPMI8226 were observed between NK cells expanded from two blood sources. CONCLUSION: Blood retained in a disposable platelet apheresis set is a useful and convenient source for expansion of NK cells for use in cancer immunotherapy research.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Donneurs de tissus
/
Aphérèse
/
Plaquettes
/
Cellules tueuses naturelles
/
Interleukine-2
/
Interleukine-15
/
Cellules K562
/
Ligand de 4-1BB
/
Immunothérapie
Limites du sujet:
Humans
langue:
Ko
Texte intégral:
Korean Journal of Blood Transfusion
Année:
2012
Type:
Article