Progress in gene knockout mice / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 784-794, 2019.
Article
Dans Chinois
| WPRIM
| ID: wpr-771331
ABSTRACT
The establishment and development of gene knockout mice have provided powerful support for the study of gene function and the treatment of human diseases. Gene targeting and gene trap are two techniques for generating gene knockout mice from embryonic stem cells. Gene targeting replaces endogenous knockout gene by homologous recombination. There are two ways to knock out target genes promoter trap and polyA trap. In recent years, many new gene knockout techniques have been developed, including Cre/loxP system, CRISP/Cas9 system, latest ZFN technology and TALEN technology. This article focuses on the several new knockout mouse techniques.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Souris knockout
/
Ciblage de gène
/
Modèles animaux de maladie humaine
/
Cellules souches embryonnaires
/
Techniques de knock-out de gènes
/
Recombinaison homologue
Limites du sujet:
Animaux
/
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2019
Type:
Article
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