Your browser doesn't support javascript.
loading
Construction and application of mCherry red fluorescent protein fusion expression system in lactic acid bacteria / 生物工程学报
Chinese Journal of Biotechnology ; (12): 492-504, 2019.
Article Dans Chinois | WPRIM | ID: wpr-771358
ABSTRACT
In order to enrich the library of domestic research about new red fluorescent marker in lactic acid bacteria (LAB), we described a new fusion expression system in Lactobacillus plantarum WCFS1 based on the pSIP vector. This system contained red fluorescent protein mCherry as a marker and bile salt hydrolase gene (bsh) as a reporter gene. Moreover, in this study, four different promoters (PsppA, PldhL, P32 and PslpA) were used to regulate the expression of the fusion protein mCherry-BSH, completing the inducible and constitutive expression in lactic acid bacteria. The recombinant protein mCherry-BSH presented activity of red fluorescence and bile salt hydrolase (BSH). The successful construction of the fusion expression system in LAB using a red fluorescent protein mCherry provides favorable conditions for the distribution, intestinal colonization and survival rate of lactic acid bacteria, so as to reveal the function mechanism of its probiotic characteristics; and the system also could lay the foundation for researches on protein expression, cellular localization and properties identification of active protein in lactic acid bacteria.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Probiotiques / Lactobacillus plantarum / Protéines luminescentes Type d'étude: Étude pronostique langue: Chinois Texte intégral: Chinese Journal of Biotechnology Année: 2019 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Probiotiques / Lactobacillus plantarum / Protéines luminescentes Type d'étude: Étude pronostique langue: Chinois Texte intégral: Chinese Journal of Biotechnology Année: 2019 Type: Article