Methods for mammalian single cell research - a review / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 27-39, 2019.
Article
Dans Chinois
| WPRIM
| ID: wpr-771403
ABSTRACT
Basic research in life science and medicine has dug into single cell level in recent years. Single-cell analysis offers to understand life from diverse perspectives and is used to profile cell heterogeneity to investigate mechanism of diseases. Single cell technologies have also found applications in forensic medicine and clinical reproductive medicine, while the techniques are rapidly evolving and have become more and more sophisticated. In this article, we reviewed various single cell isolation techniques and their pros and cons, including manual cell picking, laser capture microdissection and microfluidics, as well as analysis methods for DNA, RNA and protein in single cell. In addition, we summarized major up-to-date single cell research achievements and their potential applications.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
ADN
/
ARN
/
Séparation cellulaire
/
Analyse sur cellule unique
/
Microdissection au laser
Type d'étude:
Guide de pratique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2019
Type:
Article
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