Increased Expression of miR-146a in Children With Allergic Rhinitis After Allergen-Specific Immunotherapy
Allergy, Asthma & Immunology Research
; : 132-140, 2016.
Article
de En
| WPRIM
| ID: wpr-77209
Bibliothèque responsable:
WPRO
ABSTRACT
PURPOSE: MicroRNAs (miRs) were recently recognized to be important for immune cell differentiation and immune regulation. However, whether miRs were involved in allergen-specific immunotherapy (SIT) remains largely unknown. This study sought to examine changes in miR-146a and T regulatory cells in children with persistent allergic rhinitis (AR) after 3 months of subcutaneous immunotherapy (SCIT) and sublingual immunotherapy (SLIT). METHODS: Twenty-four HDM-sensitized children with persistent AR were enrolled and treated with SCIT (n=13) or SLIT (n=11) for 3 months. Relative miR-146a and Foxp3 mRNA expression, the TRAF6 protein level, and the ratio of post-treatment to baseline IL-10+CD4+ T cells between the SCIT and SLIT groups were examined in the peripheral blood mononuclear cells (PBMCs) of AR patients using quantitative reverse transcription polymerase chain reaction (qRT-PCR), flow cytometry, and Western blot analysis, respectively. Serum levels of IL-5 and IL-10 were determined using ELISA. RESULTS: After 3 months of SIT, both the TNSS and INSS scores were significantly decreased compared to the baseline value (P<0.01). The relative expression of miR-146a and Foxp3 mRNA was significantly increased after both SCIT and SLIT (P<0.01). The ratio of post-treatment to baseline IL-10+CD4+ T cells and the serum IL-10 level were significantly increased in both the SCIT and SLIT groups (P<0.01), whereas the TRAF6 protein level and serum IL-5 level were significantly decreased (P<0.01). No significant differences in these biomarkers were observed between the SCIT and SLIT groups. CONCLUSIONS: Our findings suggest that miR-146a and its related biomarkers may be comparably modulated after both SCIT and SLIT, highlighting miR-146a as a potential therapeutic target for the improved management of AR.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
ARN messager
/
Test ELISA
/
Lymphocytes T
/
Marqueurs biologiques
/
Différenciation cellulaire
/
Rhinite
/
Technique de Western
/
Réaction de polymérisation en chaîne
/
Interleukine-5
/
Interleukine-10
Limites du sujet:
Child
/
Humans
langue:
En
Texte intégral:
Allergy, Asthma & Immunology Research
Année:
2016
Type:
Article