Transient receptor potential Vanilloid 1-based gene therapy alleviates orthodontic pain in rats / 国际口腔科学杂志·英文版
International Journal of Oral Science
;
(4): 11-11, 2019.
Article
Dans Anglais
| WPRIM
| ID: wpr-772272
ABSTRACT
Orthodontic pain that is induced by tooth movement is an important sequela of orthodontic treatment and has a significant effect on patient quality of life. Studies have shown that the high expression of transient receptor potential vanilloid 1 (TRPV1) in trigeminal ganglions plays a vital role in the transmission and modulation of orofacial pain. However, little is known about the role of TRPV1 in orthodontic pain. In this study, male Sprague-Dawley rats were randomly assigned to six groups to study the role of TRPV1 in the modulation of tooth-movement pain. The expression levels of TRPV1 mRNA and protein were determined by real-time PCR and western blot, respectively. Moreover, pain levels were assessed using the rat grimace scale (RGS). The role of TRPV1 in modulating tooth-movement pain was examined by injecting a TRPV1 antagonist into the trigeminal ganglia of rats. A lentivirus containing a TRPV1 shRNA sequence was constructed and transduced into the rats' trigeminal ganglia. The results showed that the expression levels of TRPV1 protein and mRNA were elevated following tooth-movement pain. Pain levels increased rapidly on the 1 day, peaked on the 3 day and returned to baseline on the 14 day. The TRPV1 antagonist significantly reduced tooth-movement pain. The lentivirus containing a TRPV1 shRNA sequence was able to inhibit the expression of TRPV1 and relieved tooth-movement pain. In conclusion, TRPV1-based gene therapy may be a treatment strategy for the relief of orthodontic pain.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Douleur
/
Qualité de vie
/
Mouvement dentaire
/
Thérapie génétique
/
Répartition aléatoire
/
Peptide relié au gène de la calcitonine
/
Ganglion trigéminal
/
Rat Sprague-Dawley
/
Canaux cationiques TRPV
/
Molaire
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
International Journal of Oral Science
Année:
2019
Type:
Article
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