Effects of large tumor suppressor homolog 2 gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 609-612, 2018.
Article
Dans Chinois
| WPRIM
| ID: wpr-772450
ABSTRACT
OBJECTIVE@#To investigate the effect of large tumor suppressor homolog 2 (LATS2) gene overexpression on the proliferation and apoptosis of oral squamous cell carcinoma (OSCC).@*METHODS@#Lentivirous particles were transferred into SCC-25 cell to upregulate LATS2 gene expression. Cell proliferation was detected by CCK-8 assay. Apoptosis was detected through flow cytometry. The expression changes of Bax, Bcl-2, and LATS2 were analyzed by Western blot.@*RESULTS@#Gene transfection increased LATS2 expression. Compared with the control group and pEGFP-control group, SCC-25 cell proliferation in the pGFP-LATS2 group was inhibited, whereas the apoptosis ratio increased (P<0.05). Bcl-2 expression decreased, and Bax expression increased.@*CONCLUSIONS@#Overexpression of LATS2 could inhibit SCC-25 cell proliferation and induce apoptosis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Tumeurs de la bouche
/
Carcinome épidermoïde
/
Régulation de l'expression des gènes tumoraux
/
Protein-Serine-Threonine Kinases
/
Apoptose
/
Protéines suppresseurs de tumeurs
/
Lignée cellulaire tumorale
/
Prolifération cellulaire
/
Génétique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
West China Journal of Stomatology
Année:
2018
Type:
Article
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