A non-coated enzyme-linked immunosorbent assay for screening zika virus envelope protein / 南方医科大学学报

ABSTRACT

OBJECTIVE@#To establish a non-coated enzyme-linked immunosorbent assay (ELISA) based on zika virus envelope (E) protein for detecting the expression of E protein in infected cells.@*METHODS@#Adherent Vero-143 cells infected with zika virus in a 96-well plate were fixed, and the antibodies against zika virus E protein were added at an optimized concentration to establish the non-coated ELISA method for E protein. The antiviral activities of lignans compound C1 was evaluated using this method. The accuracy of this non-coated ELISA was verified by RT-PCR, and the cross reaction with dengue virus was assessed.@*RESULTS@#After optimization, the background absorbance at 450 nm of uninfected cells was reduced to about 0.20. The antiviral activities of lignans compound C1 detected by this method were basically consistent with the results of RT-PCR. No cross reaction with dengue virus was found in this assay.@*CONCLUSIONS@#A non- coated ELISA method based on zika virus E protein was established, which can be used for screening antiviral agents against zika virus.