Osthole decreases collagen I/III contents and their ratio in TGF-β1-overexpressed mouse cardiac fibroblasts through regulating the TGF-β/Smad signaling pathway / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 321-329, 2018.
Article
Dans En
| WPRIM
| ID: wpr-773610
Responsable en Bibliothèque :
WPRO
ABSTRACT
The present study was designed to elucidate whether the mechanism by which osthole decreases collagenI/III contents and their ratio is regulating the TGF-β/Smad signaling pathway in TGF-β1-overexpressed mouse cardiac fibroblasts (CFs). These CFs were cultured and treated with different concentrations of osthole. Our results showed that the TGF-β1 expression in the CFs transfected with that the recombinant expression plasmids pcDNA3.1(+)-TGF-β1 was significantly enhanced. After the CFs were treated with 1.25-5 μg·mL of osthole for 24 h, the mRNA and protein expression levels of collagensIand III were reduced. The collagen I/III ratio was also reduced. The mRNA and protein expression levels of TGF-β1, TβRI, Smad2/3, P-Smad2/3, Smad4, and α-SMA were decreased, whereas the expression level of Smad7 was increased. These effects suggested that osthole could inhibit collagen I and III expression and reduce their ratio via the TGF-β/Smad signaling pathway in TGF-β1 overexpressed CFs. These effects of osthole may play beneficial roles in the prevention and treatment of myocardial fibrosis.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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ARN messager
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Transduction du signal
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Cellules cultivées
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Régulation de l'expression des gènes
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Actines
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Collagène
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Protein-Serine-Threonine Kinases
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Récepteurs TGF-bêta
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Coumarines
Limites du sujet:
Animals
langue:
En
Texte intégral:
Chinese Journal of Natural Medicines (English Ed.)
Année:
2018
Type:
Article