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Identification of Corydalis yanhusuo,C. turtschaninovii,C. decumbens by allele-specific PCR / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 3261-3267, 2019.
Article Dans Chinois | WPRIM | ID: wpr-773724
ABSTRACT
To establish a DNA molecular markers method for identification of Corydalis yanhusuo,C. turtschaninovii and C. decumbens,the mat K,trn G and psb A-trn H sequences of 56 samples from 14 species of C. yanhusuo,C. turtschaninovii,C. decumbens and their related species were obtained by sequencing. The SNP loci were obtained by Bio Edit 7. 2. 2 software. The primers for AS-PCR identification were designed based on the mutation sites,and the conditions of PCR were optimized to identify C. yanhusuo,C. turtschaninovii,and C. decumbens according to the specific bands. The results showed that the amount of template( 0. 6-1 200 ng)and annealing temperature( 42-60 ℃) had little influence on the amplification results,and the number of cycles had much influence on the amplification results. When the number of cycles was 20,the specific bands of 297 bp( mat K),353 bp( trn G) and 544 bp( mat K) were amplified from C. yanhusuo,C. turtschaninovii and C. decumbens,respectively. The method established in this study had a minimum detection limit of 6 ng for C. yanhusuo,60 ng for C. decumbens and less than 0. 6 ng for C. turtschaninovii. Thus,the allelespecific PCR method established in the research can specifically identify C. yanhusuo,C. turtschaninovii,and C. decumbens.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Marqueurs génétiques / Réaction de polymérisation en chaîne / Classification / Gènes de plante / Corydalis / Allèles / Génétique Type d'étude: Etude diagnostique langue: Chinois Texte intégral: China Journal of Chinese Materia Medica Année: 2019 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Marqueurs génétiques / Réaction de polymérisation en chaîne / Classification / Gènes de plante / Corydalis / Allèles / Génétique Type d'étude: Etude diagnostique langue: Chinois Texte intégral: China Journal of Chinese Materia Medica Année: 2019 Type: Article