Effects of endothelial progenitor cells on proliferation and biological function of hepatic stellate cells under shear stress / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 404-407, 2018.
Article
Dans Chinois
| WPRIM
| ID: wpr-773772
ABSTRACT
OBJECTIVE@#To investigate the effects of endothelial progenitor cells (EPCs) under shear stress on the biological function such as proliferation, adhesion, migration, apoptosis and expression of α-smooth muscle actin (α-SMA), collagen-I and collagen-Ⅲ of hepatic stellate cells (HSCs).@*METHODS@#HSCs and EPCs were inoculated into the upper and lower layers of the co-culture chamber respectively and co-incubated for 24 hours. Then, 12 dyne/cm shear stress was applied to EPCs cells for another 24 hours. After that, proliferation, adhesion, migration and apoptosis of HSCs were detected by cell counting kit-8 (CCK-8) kit, cell adherent assay, Boyden cell migration assay and flow cytometry respectively. Fluorescence quantitative PCR and Western blot were used to detect the mRNA and protein expression of alpha -SMA, collagen I and collagen-Ⅲ in HSCs.@*RESULTS@#Under shear stress, EPCs ecological niche could obviously inhibit the proliferation, adhesion and migration of HSCs, promote the apoptosis of HSCs, and down-regulate the mRNA and protein expression of collagen-I, collagen-Ⅲ in HSC cells.@*CONCLUSIONS@#Under shear stress, EPCs ecological niche could inhibit the fibrosis development of HSCs to a certain extent.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Cellules cultivées
/
Actines
/
Apoptose
/
Collagène de type I
/
Prolifération cellulaire
/
Cellules étoilées du foie
/
Progéniteurs endothéliaux
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2018
Type:
Article
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