Rapid identification of chemical components in Xingbei Zhike Keli by UPLC-Q-TOF-MS/MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 4439-4449, 2018.
Article
Dans Chinois
| WPRIM
| ID: wpr-775323
ABSTRACT
To analyze and identify the chemical components in Xingbei Zhike Keli by using ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF-MS/MS). The analysis was performed on Agilent Zorbax SB-C₁₈(4.6 mm×250 mm, 5 μm) column, with methanol-0.08% formic acid solution (including 0.1% ammonium formate) as the mobile phase for gradient elution. The flow rate was 1 mL·min⁻¹ and column temperature was 30 °C. The MS spectrum was acquired in both negative and positive ion modes by using electron spray ionization (ESI). These components were further analyzed based on accurate m/z, secondary fragmentation and other information combined with reference substance and literature data. As a result, 87 compounds were successfully identified and predicted, including alkaloids, flavonoids, coumarins and saponins, of which 23 compounds were verified by comparing with reference substances. These results provide reference for the quality control of Xingbei Zhike Keli, and lay the foundation for elucidating their effective components and mechanism of action.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Flavonoïdes
/
Médicaments issus de plantes chinoises
/
Chromatographie en phase liquide à haute performance
/
Spectrométrie de masse ESI
/
Alcaloïdes
/
Spectrométrie de masse en tandem
Type d'étude:
Etude diagnostique
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2018
Type:
Article
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