Establishment of a quantitative real-time PCR for rapid detection of Panax quinquefolius / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4050-4054, 2018.
Article
Dans Zh
| WPRIM
| ID: wpr-775380
Responsable en Bibliothèque :
WPRO
ABSTRACT
In this study, the specific primers and probes of Panax quinquefolius were designed for a quantitative real-time PCR, and the rapid identification method of P. quinquefolius was established by optimizing conditions. The method was used to validate 43 samples of the traditional Chinese medicine,and the results showed that 22 samples of P. quinquefolius were identified accurately. The limit of detection of the method can be reach to 1×10⁻⁴ ng. The method is accurate, fast, sensitive and specifically.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Normes de référence
/
Médicaments issus de plantes chinoises
/
Sondes d'ADN
/
Amorces ADN
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Réaction de polymérisation en chaine en temps réel
/
Génétique
/
Panax
Type d'étude:
Diagnostic_studies
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2018
Type:
Article