Role of Reactive Oxygen Species in GDC-0152-Induced Apoptosis and Autophagy of NB4 cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1786-1793, 2019.
Article
Dans Chinois
| WPRIM
| ID: wpr-781396
ABSTRACT
OBJECTIVE@#To investigate the effect of reactive oxygen species (ROS) on GDC-0152-induced apoptosis and autophagy of acute promyelocytic leukemia cell line NB4.@*METHODS@#Different concentrations of GDC-0152 combined with Z-VAD-FMK was applied to NB4 cells. Cell proliferation was detected by CCK8 method. Apoptosis rate, autophagy and ROS level were detected by flow cytometry. The autophagy was observed by Cyto-ID staining fluorescence microscopy, and flow cytometry were used to detect the fluorescence expression. The expression of autophagy-related protein LC3B was detected by Western blot.@*RESULTS@#GDC-0152 increased proliferation inhibition rate and apoptosis rate in NB4 cells (P<0.05); GDC-0152 induced increase of ROS level of NB4 cells; GDC-0152 increased autophagy of NB4 cells that was found by Cyto-ID staining fluorescence microscopy and flow cytometry (P<0.05). Western blot showed that GDC-0152 increased LC3B expression in NB4 cells and promoted the conversion of LC3BI to LC3BII; as compared with GDC-0152 (100 ng/ml), GDC-0152 (100 ng/ml) combined with ROS inhibitor YCG063 (10 μmol/L) decreased apoptosis and autophagy (P<0.05).@*CONCLUSION@#GDC-0152 inhibits cell proliferation by inducing apoptosis and autophagy of NB4 cells. ROS can promote GDC-0152-induced apoptosis and autophagy of NB4 cells.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pyrroles
/
Autophagie
/
Leucémie aiguë promyélocytaire
/
Espèces réactives de l'oxygène
/
Apoptose
/
Cyclohexanes
/
Lignée cellulaire tumorale
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2019
Type:
Article
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