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Effects of Peroxisome Proliferator-Activated Receptor-gamma on the Production of Tumor Necrosis Factor-alpha in Stimulated Human Monocoytes / 감염과화학요법
Infection and Chemotherapy ; : 291-295, 2010.
Article Dans Coréen | WPRIM | ID: wpr-78360
ABSTRACT

BACKGROUND:

We evaluated the effects of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) on the production of tumor necrosis factor-alpha (TNF-alpha) and expression of nuclear factor-kappaB (NF-kappaB) in stimulated THP-1 cells, a human monocyte cell line. MATERIALS AND

METHODS:

We evaluated the cytotoxic effect of 15-Deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), one of natural PPAR-gamma ligands, using commercial cell proliferation assay. Cells were pretreated with 15d-PGJ(2) and then stimulated with lipopolysaccharide (LPS) or lipoteichoic acid (LTA). The amount of TNF-alpha was measured by using commercial ELISA method. NF-kappaB activation was evaluated by Western blot analysis.

RESULTS:

15d-PGJ(2) showed dose-dependent cytotoxic effect on the tested cells after 4 hr of treatment. Stimulation of cells by LPS or LTA induced TNF-alpha production. TNF-alpha production was markedly decreased in the cells pretreated with 15d-PGJ(2) compared to cells treated only with LPS or LTA in a dose-dependent manner. Pretreatment of 15d-PGJ(2) reduced LPS or LTA induced NF-kappaB expression in the nuclear extracts of THP-1 cells.

CONCLUSION:

15d-PGJ(2) pretreatment decreased TNF-alpha production from the THP-1 cells stimulated by LPS or LTA, and this assumed to be associated with inhibition of NF-kappaB activation.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Acides teichoïques / Test ELISA / Monocytes / Lignée cellulaire / Lipopolysaccharides / Technique de Western / Facteur de transcription NF-kappa B / Facteur de nécrose tumorale alpha / Péroxysomes / Prolifération cellulaire Limites du sujet: Humains langue: Coréen Texte intégral: Infection and Chemotherapy Année: 2010 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Acides teichoïques / Test ELISA / Monocytes / Lignée cellulaire / Lipopolysaccharides / Technique de Western / Facteur de transcription NF-kappa B / Facteur de nécrose tumorale alpha / Péroxysomes / Prolifération cellulaire Limites du sujet: Humains langue: Coréen Texte intégral: Infection and Chemotherapy Année: 2010 Type: Article