Expression of Nitric Oxide Synthase and Aquaporin-3 in Cyclophosphamide Treated Rat Bladder / 대한배뇨장애요실금학회지
International Neurourology Journal
;
: 149-156, 2010.
Article
Dans Anglais
| WPRIM
| ID: wpr-78370
ABSTRACT
PURPOSE:
The expression of Nitric oxide Synthase (NOS) and aquaporin (AQP) water channels in rat bladder is recently reported. The aim of this study is to evaluate the expression of inducible NOS (iNOS), aquaporin-3 (AQP-3) in cyclophosphamide (CYP) induced rat bladder. MATERIALS ANDMETHODS:
The 32 Sprague-Dawley rats were divided into cystitis group (n=20) and control group (n=12). In cystitis group, 100mg/kg CYP was injected every second day for 1 week whereas in control group, normal saline was injected. After extracting of the bladder and dividing dome, body and trigone of the bladder, independently H&E staining and immunohistochemical staining for iNOS and AQP-3 were performed. Expressions of iNOS and AQP-3 were analyzed with a confocal laser scanning microscope and an image analyzer.RESULTS:
The expression of iNOS significantly increased in the mucosa, submucosa layer of dome in cystitis group (p<0.05). The expression of AQP-3 significantly increased in the mucosa, submucosa, vessel layer of dome in cystitis group (p<0.05).CONCLUSIONS:
These results suggest that inflammatory change activates NOS and AQP-3 expression in the bladder tissue of rats. These may imply that NOS and AQP-3 have a pathophyiological role in the cyclophophamide induced interstitial cystitis. Further study on the NOS and AQP-3 in bladder is needed for clinical application.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Vessie urinaire
/
Rat Sprague-Dawley
/
Nitric oxide synthase
/
Cystite interstitielle
/
Aquaporines
/
Cyclophosphamide
/
Cystite
/
Glycosaminoglycanes
/
Muqueuse
/
Monoxyde d'azote
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
International Neurourology Journal
Année:
2010
Type:
Article
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