Perivascular Stem Cells Suppress Inflammasome Activation during Inflammatory Responses in Macrophages
International Journal of Stem Cells
;
: 419-429, 2019.
Article
Dans Anglais
| WPRIM
| ID: wpr-785832
ABSTRACT
BACKGROUND AND OBJECTIVES:
Perivascular stem cells (PVCs) have been identified as precursors of mesenchymal stem cells (MSCs) that offer promising prospects for application in the development of cellular therapies. Although PVCs have been demonstrated to have greater therapeutic potential compared to bone marrow and adipose tissue-derived MSCs in various diseases, the regulatory role of PVCs on inflammasome activation during macrophage-mediated inflammatory responses has not been investigated.METHODS ANDRESULTS:
In this study, we found that the PVC secretome effectively alleviates secretion of both caspase-1 and interleukin-1β in lipopolysaccharide-primed and activated human and murine macrophages by blocking inflammasome activation and attenuating the production of mitochondrial reactive oxygen species (ROS). We further showed that the PVC secretome significantly reduces inflammatory responses and endoplasmic reticulum stress in peritoneal macrophages in a mouse model of monosodium urate-induced peritonitis. A cytokine antibody array analysis revealed that the PVC secretome contains high levels of serpin E1 and angiogenin, which may be responsible for the inhibitory effects on mitochondrial ROS generation as well as on inflammasome activation.CONCLUSIONS:
Our results suggest that PVCs may be therapeutically useful for the treatment of macrophage- and inflammation-mediated diseases by paracrine action via the secretion of various biological factors.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Péritonite
/
Cellules souches
/
Moelle osseuse
/
Facteurs biologiques
/
Espèces réactives de l'oxygène
/
Inhibiteur-1 d'activateur du plasminogène
/
Macrophages péritonéaux
/
Inflammasomes
/
Cellules souches mésenchymateuses
/
Stress du réticulum endoplasmique
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
International Journal of Stem Cells
Année:
2019
Type:
Article
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