Inactivation and validation of virus in blood products of human coagulation fac-torⅧ / 药学实践杂志
Journal of Pharmaceutical Practice
; (6): 199-202, 2014.
Article
de Zh
| WPRIM
| ID: wpr-790313
Bibliothèque responsable:
WPRO
ABSTRACT
Objective To study effect of virus inactivation/removal treated by solvent/detergent method and dry heating at 80℃, 72 h for inactivation in human coagulation factor Ⅷ.Methods Human coagulation factor Ⅷextracted from healthy human plas-ma were treated by solvent/detergent method and dry heating at 80℃, 72 h for inactivation .The virus inactivation effect was validated by adding the indicator virus ( PRV, Sindbis, HIV, EMCV, PPV).Results The methods could effectively inactivate lipid-enveloped and non lipid-enveloped viruses which could be used for virus inactivation /removal during human coagulation factor Ⅷexperiments , the residual amount of TNBP in production was less than one percent ten thousand (10 ppm), the residual Tween-80 concentration was less than one percent hundred thousand (100 ppm),which all met the safety standards .Conclusion and no significant change was ob-served in the activation and other indicators of human coagulation factor Ⅷ.
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Indice:
WPRIM
langue:
Zh
Texte intégral:
Journal of Pharmaceutical Practice
Année:
2014
Type:
Article