Study on synthesis and cyto-toxicity of polyethylene glycol-b-polylysine as poten-cial miRNA carrier / 药学实践杂志
Journal of Pharmaceutical Practice
;
(6): 362-365,373, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-790362
ABSTRACT
Objective To synthesize miRNA carrier PEG-b-PLL and to testify the stability , encapsulation efficiency and cyto-toxicity of its complexes .Methods H1 NMR was used to determine the degree of polymerization of PLL , 4%agarose gel electrophore-sis was used to determine entrapment of the polyer to the miRNA;then dynamic light scattering ( DLS) was used to measure the hydro-dynamic parameter such as size , polydispersity index ( PDI) and zeta potential of the polyplexes .The entrapment efficiency was deter-mined by ultraviolet-visible spectrophotometer , and finally , the cyto-toxicity of PEG-b-PLL was evaluated by CKK-8 kit with K562 cell lines.Results The characteristics indicated polyplexes prepared by PEG-b-PLL and miRNA fulfill the demand of being the gene carri-er of miRNA because of low cyto-toxicity , high encapsulation efficiency and stability .Conclusion The miRNA carrier PEG-b-PLL had good character and low cyto-toxicity.It showed considerable potential as an efficient miRNA carrier .
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Journal of Pharmaceutical Practice
Année:
2014
Type:
Article
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