Simultaneous determination of four ingredients in Gan Bawei capsule by HPLC-DAD / 药学实践杂志

ABSTRACT

Objective To establish an HPLC-DAD method for simultaneous determination of four ingredients in Gan Ba-wei capsule .Methods HPLC method was adopted with C18 column(250 mm × 4.6 mm ,5 μm) ,Acetonitrile as mobile phase A and water as mobile phase B in gradient elution mode . The detection wavelength was 240 nm and the flow rate was 1.0 ml/min .Results Salvianolic acid B reference substance was excellently linear in the range of 15.026~100.17 μg/ml ,the average recovery was 96.6% (n=6)and RSD was 1.8% (n=6) .Paeoniflorin reference substance was excellently linear in the range of 14.335~95.564 μg/ml ,the average recovery was 97.0% (n=6)and RSD was 1.7% (n=6) .Polydatin reference sub-stance was excellently linear in the range of 8.235-54.90 μg/ml ,the average recovery was 97.2% (n=6)and RSD was 1.8%(n=6) .Emodin reference substance was excellently linear in the range of 1.582 5-10.55 μg/ml ,the average recovery was 98.1% (n=6)and RSD was 1.7% (n=6) .Conclusion The method was easy ,accurate ,reproducible ,specific and without in-terference in negative control ,which could be used for quality control of Gan Bawei capsule .